Zuhair K. Ballas, MD, Director
Professor, Department of Internal Medicine
Chief of the Allergy and Immunology Division
Justin Fishbaugh, Engineer III
Technical Director
Burl E. Hess, Engineer I
Research Assistant
Core Facility Office: 48 EMRB
Telephone: 319-335-8103
Fax: 319-335-8104
Hours of Operation:
8 a.m. - 8 p.m. Monday through Friday
The facility can be accessed after hours for data analysis.
Services provided by the core include multicolor flow
cytometric analysis based on surface markers, cytoplasmic
markers, and nucleic acid staining, and sterile sorting of
live cell populations.
Purpose:
- Provide advice and technical expertise for analysis
and/or separation of cell populations based upon their
ability to bind fluorochrome-conjugated antibodies.
- Aid investigators in the use of the EPICS 753 flow
cytometer for analysis and sorting for cell
populations.
- Train investigators and their laboratory personnel in
the use of the FACScan for analysis of cell
populations.
- Train and assist investigators in the use of the
facility's computers for the analysis and output of data
accumulated during the flow cytometry studies.
- Aid investigators in the use and/or development of
new fluorochromes for analysis of cells populations.
Services Provided to Cancer Center
Members:
- Assessment of cell size and granularity
- Distinction between viable and dead cells
- Surface density of up to four surface markers or
antigens plus forward and orthogonal scatter
- Cell cycle analysis
- Analysis of up to 2 surface markers and DNA (cell
cycle)
- Analysis of programmed cell death (apoptosis)
- Analysis of cytoplasmic proteins (e.g. cytokines, T
cell receptors)
- Simultaneous analysis of three cell surface markers
and one cytoplasmic marker
- Analysis of intracellular calcium flux vs. time
- Simultaneous analysis of two cell surface markers and
intracellular calcium flux vs. time
- Superoxide generation
- Cell sorting
- Chromosomal analysis
- Indefinite archiving of data
- Data analysis
- Preparation of publication quality computer
graphics
- Consultation as to experimental design
- Consultation as to advantages and disadvantages of
various fluorochromes and specific antibodies
- Autocloning (this allows the sorting of as little as
a single cell which is deposited in an individual well of
a microtiter plate)
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