Daniel Bonthius, MD, PhD
Research Profile
Research Interests:
My research interests are central nervous system development and the factors that can disrupt it. I focus my research on two neuroteratogenic agents: alcohol (fetal alcohol syndrome) and lymphocytic choriomeningitis virus (congenital LCMV infection). I am currently investigating the cellular and molecular mechanisms underlying alcohol- and LCMV-induced injury to the developing brain, the behavioral consequences of these injuries, and therapeutic agents to minimize or reverse the injuries.
I am also interested in the development of gene therapy for neurological diseases. In particular, I am exploring the use of viral gene therapy vectors for the treatment of Alexander Disease, a devastating genetic disease of the pediatric brain. |
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Lab Members:
- Daniel Bonthius, MD, PhD
- Bahri Karacay, PhD
- Glenda Rabe, MD
- Shenglan Li, BS, MA
- Jo Mahoney, BS
- Hannah Klein de Licona, BS
- Mark Luong
Affiliations:
- Neuroscience Program
- Medical Scientist Training Program
- Department of Neurology
- Cellular and Molecular Biology Program
Model System:
We utilize animal model systems and tissue culture
systems of fetal alcohol syndrome, congenital LCMV infection, and
Alexander Disease.
Recent Publications:
- Daniel J. Bonthius, Bahri Karacay, Jolonda C. Mahoney, Michael J. Buchmeier and Derek A. Taggard. Critical role for glial cells in the propagation and spread of lymphocytic choriomeningitis virus in the developing rat brain. Journal of Virology 76: 6618-6635, 2002.
- Charles Grose, Daniel Bonthius and Adel K. Afifi. Chickenpox and the geniculate ganglion: facial nerve palsy, Ramsay Hunt syndrome and acyclovir treatment. Pediatric Infectious Disease Journal 21: 615-617, 2002.
- Linda W. Dickerson, Daniel J. Bonthius, Brian C. Schutte, Baoli Yang, Thomas J. Barna, Melissa C.Bailey, Keith Nehrke, Roger A. Williamson, and Fred Lamb. Altered GABAergic function accompanies hippocampal degeneration in mice lacking ClC-3 voltage-gated chloride channels. Brain Research 958: 227-250, 2002.
- Daniel J. Bonthius, Georgios Tzouras, Bahri Karacay, Jolonda Mahoney, Ana Hutton, Ross McKim, and Nicholas J. Pantazis. Deficiency of neuronal nitric oxide synthase (nNOS) worsens alcohol-induced microencephaly and neuronal loss in developing mice. Developmental Brain Research 138: 45-59, 2002.
- Daniel J. Bonthius, Bahri Karacay, De Dai, and Nicholas J. Pantazis. FGF-2, NGF and IGF-1, but not BDNF, utilize a nitric oxide pathway to signal neurotrophic and neuroprotective effects against alcohol toxicity in cerebellar granule cell cultures. Developmental Brain Research 140: 15-28, 2003.
- Bahri Karacay, M. Sue O’Dorisio, Monica Summers, Michael Robinson and Daniel J. Bonthius. Expression of VIP Receptor 1 (VPAC1) promoter in central and peripheral nervous system. Regulatory Peptides 116: 1-12, 2003.
- Daniel J. Bonthius and Bahri Karacay. Sydenham’s chorea: not gone and not forgotten. Seminars in Pediatric Neurology 10: 11-19, 2003.
- Daniel J. Bonthius, Bahri Karacay, De Dai, Ana Hutton, and Nicholas J. Pantazis. The NO-cGMP-PKG pathway plays an essential role in the acquisition of ethanol resistance by cerebellar granule neurons. Neurotoxicology and Teratology 26: 47-57, 2004.
- Bahri Karacay, Salih Sanlioglu, Thomas S. Griffith, Anthony Sandler, and Daniel J. Bonthius. Inhibition of the NF-kB pathway enhances TRAIL-mediated apoptosis in neuroblastoma cells. Cancer Gene Therapy 11: 681-690, 2004.
- Daniel J. Bonthius, Ross McKim, Lindsey Koele, Harb Harb, Bahri Karacay, Jo Mahoney and Nicholas J. Pantazis. Use of frozen sections to determine neuronal number in the murine hippocampus and neocortex using the optical disector and optical fractionator. Brain Research Protocols 14: 45-57, 2004.
- Daniel J. Bonthius, Ana Solodkin, and Gary W. Van Hoesen. Pathology of the insular cortex in Alzheimer’s disease depends on cortical architecture. Journal of Neuropathology and Experimental Neurology 64: 910-922, 2005.
- Dennis C. Harper, Hanes M. Swingle, Carl P. Weiner, Daniel J. Bonthius, Glen P. Aylward, and John A. Widness. Long-term neurodevelopmental outcome and brain volumes of children following severe fetal anemia with hydrops. American Journal of Obstetrics and Gynecology, 195: 192-200, 2006.
- Daniel J. Bonthius, Ross A. McKim, Lindsey Koele, Harb Harb, Ana Hutton Kehrberg, Jo Mahoney, Bahri Karacay, Nicholas J. Pantazis. Severe alcohol-induced neuronal deficits in the hippocampus and neocortex of neonatal mice genetically deficient for neuronal nitric oxide synthase (nNOS). Journal of Comparative Neurology, 499: 290-305, 2006.
- Daniel J. Bonthius, Heather Carmichael Olson, and Jennifer D. Thomas. Proceedings of the 2006 annual meeting of the Fetal Alcohol Spectrum Disorders Study Group. Alcohol - An International Biomedical Journal 40: 61-65, 2006.
- Bahri Karacay, Guiying Li, Nicholas J. Pantazis and Daniel J. Bonthius. Stimulation of the cAMP pathway protects cultured cerebellar granule neurons against alcohol-induced cell death by activating the neuronal nitric oxide synthase (nNOS) gene. Brain Research, 1143: 34-45, 2007.
- Daniel J. Bonthius, Rhonda Wright, Brian Tseng, Leslie Barton, Elysa Marco, Bahri Karacay, and Paul D. Larsen. Congenital lymphocytic choriomeningitis virus infection: Spectrum of disease. Annals of Neurology, 62: 347-355, 2007.
- Daniel J. Bonthius, Brent Nichols, Harb Harb, Jo Mahoney, and Bahri Karacay. Lymphocytic choriomeningitis virus infection in brain: Critical role of host age. Annals of Neurology, 62: 356-374, 2007.
- Andrew Lee, Richard J. Olson, and Daniel J. Bonthius. Increasing exotropia and decreasing vision in a school-aged boy. Survey of Ophthalmology, in press, 2007.
- Daniel J. Bonthius and Stanley Perlman. Congenital viral infections of the brain: lessons learned from lymphocytic choriomeningitis virus in the neonatal rat. PLoS Pathogens, in press, 2007.
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