Paul B. McCray, MD
Research Profile

Lab Members:

Paul McCray, Risa Banasik, Jennifer Bartlett, Doug Dylla, Tony Fischer, Ben Hanshaw, Melissa Hickey, Hongpeng Jia, Cameron Kiersch, Michael Leinen, Patrick Sinn, Tim Starner, Ping Tan, Chris Wohlford-Lenane, Litao Xie, Niu Zhang, Weiqiang Zhou.

Research Lab

Affiliations:

• Department of Pediatrics
• MSTP
• Genetics PhD Program

Model System:

• In vitro cell culture models
• Small animal models

Recent Publications (past 3 years):

1. Sinn PL, Hickey MA, Staber PD, Dylla DE, Jeffers SA, Davidson BL, Sanders DA, McCray PB Jr., Lentivirus vectors pseudotyped with filoviral envelope glycoproteins transduce airway epithelia from the apical surface independent of folate receptor-alpha. J Virol 77:5902-5910, 2003.
2. Brogden KA, Heidari M, Sacco RE, Palmquist D, Guthmiller JM, Johnson GK, Jia HP, Tack BF, McCray PB Jr., Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease. Oral Microbiol Immunol 18:95-99, 2003.
3. Taggart CC, Greene CM, Smith SG, Levine RL, McCray PB Jr., O'Neill S, McElvaney NG. Inactivation of human beta-defensin 2 and 3 by elastolytic cathepsins. J Immunol 171:931-937, 2003.
4. Brogden KA, Ackermann M, McCray PB Jr., Tack BF. Antimicrobial peptides in animals and their role in host defenses. Int J Antimicrob Agents 22:465-478, 2003.
5. Starner TD, Barker CL, Jia HP, Kang Y, McCray PB Jr., CCL20 is an inducible product of human airway epithelia with innate immune properties. Am J Respir Cell Mol Biol 29:627-633, 2003.
6. Bartlett KH, McCray PB Jr., Thorne PS. Novispirin G10-induced lung toxicity in a Klebsiella pneumoniae infection model. Antimicrob Agents Chemother 47:3901-3906, 2003.
7. Sinn PL, McCray PB Jr., Cells of respiratory epithelium. Methods Mol Biol 229:287-298, 2003.
8. Meyerholz DK, Gallup JM, Grubor BM, Evans RB, Tack BF, McCray PB Jr., Ackermann MR. Developmental expression and distribution of sheep beta-defensin-2. Dev Comp Immunol 28:171-178, 2004.
9. Premratanachai P, Joly S, Johnson GK, McCray PB Jr., Jia HP, Guthmiller JM. Expression and regulation of novel human β-defensins in gingival keratinocytes. Oral Microbiol Immunol 19:111-117, 2004.
10. Joly S, Maze C, McCray PB Jr., Guthmiller JM. Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. J Clin Microbiol 42:1024-1029, 2004.
11. Jia HP, Kline JN, Penisten A, Apicella MA, Gioannini TL, Weiss J, McCray PB Jr. Endotoxin responsiveness of human airway epithelia is limited by low expression of MD 2. Am J Physiol, Lung Cell Mol Physiol 287:L428-L437, 2004.
12. Bartlett KH, McCray PB Jr., Thorne PS. Reduction in the bactericidal activity of selected cathelicidin peptides by Bovine calf serum or exogenous endotoxin. Int J Antimicrob Agents 23:606-612, 2004.
13. Jia HP, Starner TD, Bartlett J, McCray PB Jr., The antimicrobial chemical shield in CF - Chinks in the armor. Pediatr Pulmonol, Suppl 27:99;S2.2, 2004.
14. Scheetz TE, Zabner J, Welsh MJ, Coco J, Eyestone M, de Fatima Bonaldo M, Kucaba T, Casavant TL, Soares MB, McCray PB Jr., Large scale gene discovery in human airway epithelia reveals novel transcripts. Physiol Genomics 17:69-77, 2004.
15. Bissell J, Joly S, Johnson GK, Organ C, Dawson D, McCray PB Jr., Guthmiller JM. Expression of beta-defensins in gingival health and in periodontal disease. J Oral Pathol Med 33:278-285, 2004.
16. Starner TD, Agerberth B, Gudmundsson, GH, McCray PB Jr., Expression and activity of beta-defensins and LL37 in the developing human lung. J Immunol 174:1608-1615, 2005.
17. Sinn PL, Shah AJ, Donovan MD, McCray PB Jr.. Viscoelastic gel formulations enhance airway epithelial gene transfer with viral vectors. Am J Respir Cell Mol Biol 32:404-410, 2005.
18. Sinn PL, Burnight ER, Shen H, Fan H, McCray PB Jr., Inclusion of Jaagsiekte sheep retrovirus proviral elements markedly increases lentivirus vector pseudotyping efficiency. Mol Ther 11:460-469, 2005.
19. Joly S, Organ CC, Johnson GK, McCray PB Jr., Guthmiller JM. Correlation between beta-defensin expression and induction profiles in gingival keratinocytes. Mol Immunol 42:1073-1084, 2005.
20. Sinn PL, Penisten AK, Burnight ER, Hickey MA, Williams G. McCoy DM, Mallampalli RK, McCray PB Jr., Gene transfer to respiratory epithelia with lentivirus pseudotyped with Jaagsiekte sheep retrovirus envelope glycoprotein. Hum Gene Ther 16:479-488, 2005.
21. Sinn PL, Sauter SL, McCray PB Jr., Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors - design, biosafety, and production. Gene Ther 12:1089-1098, 2005.
22. Kang Y, Xie L, Tran DT, Stein CS, Hickey M, Davidson BL, McCray PB Jr., Persistent expression of factor VIII in vivo following nonprimate lentiviral gene transfer. Blood 106:1552-1558, 2005.
23. Sinn PL, Burnight ER, Hickey MA, Blissard GW, McCray PB Jr., Persistent gene expression in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transfer. J Virol 79:12818-12827, 2005.
24. Zabner J, Scheetz TL, Abdulkawy H, Casavant T, Welsh MJ, Huang J. McCray PB Jr., The CFTR deltaF508 mutation has minimal effect on the gene expression profile of differentiated human airway epithelia. Am J Physiol Lung Cell Mol Physiol 289:L545-L553, 2005.
25. Jia H, Look D, Shi L, Hickey M, Pewe L, Netland J, Farzan M, Wohlford-Lenane C, Perlman S, McCray PB Jr., ACE2 receptor expression and severe acute respiratory syndrome (SARS) coronavirus infection depend on differentiation of human airway epithelia. J Virol 79:14614-14621, 2005.
26. Starner TD, Zhang N, Kim G, Apicella MA, McCray PB Jr., Haemophilus influenzae forms biofilms on airway epithelia: Implications in cystic fibrosis. Am J Respir Crit Care Med, May 4, 2006 [Epub ahead of print].
27. Kang Y, Moressi CJ, Scheetz TE, Xie L, Tran DT, Casavant TL, Ak P, Benham CJ, Davidson BL, McCray PB Jr., Integration site choice of a feline immunodeficiency virus vector. J Virol 80:8820-8823, 2006.
28. Meyerholz DK, Grubor B, Lazic T, Derscheid RJ, Gallup JM, McCray PB Jr., Ackermann MR. Monocytic/macrophagic pneumonitis following intrabronchial deposition of vascular endothelial growth factor in neonatal lambs. Veterinary Pathology. (in press)
29. Flotte TR, Ng P, Dylla D, McCray PB Jr., Wang G, Kolls JK, Hu J. Gene and cell therapies for treatment of cystic fibrosis. Mol Ther. (in press)
30. Viriyakosol S, McCray PB Jr., Ashbaugh MA, Chu J, Jia HP, Weiss J, Kirkland TN. Characterization of monoclonal antibodies to human soluble MD-2. Hybridoma. (in press)