Garry R. Buettner, PhD
Professor and Assistant Director, Free Radical and Radiation Biology
Graduate Program and Director, ESR Facility
Buettner Lab Information
Contact Information
Free Radical Radiation Biology
ESR Facility
Carver College of Medicine
EMRB 68
The University of Iowa
Iowa City, IA 52242-1101, USA
Tel: 319-335-6749
Fax:319-335-8039
Email: garry-buettner@uiowa.edu
Research Interests
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The fundamental chemistry and biochemistry of vitamins C and E.
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Nitric Oxide is being investigated (NIH funded) as an important
membrane antioxidant in cells and tissue. We hypothesize that it is
as important as Vitamin E.
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The use of the ascorbate radical as a marker for free radical oxidative
stress. This research has provided the foundation for many ongoing
free radical-related research projects here at Iowa and around the
world.
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Nitric oxide production during stress. We are developing methods
to monitor NO-production in vivo. We have demonstrated that
NO is produced during heat stress and we have now extended this to
local hyperthermia. We have extended this research (NIH-funded) into
the world of hyperthermia as used in the treatment of cancer. We have
already demonstrated that local hyperthermia of tumor tissue results
in both free-radical and NO-production.
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Redox state - This research effort has as a goal to arrive at a
quantitative measurement of the redox environment of a cell/tissue.
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Free radicals in membrane lipid peroxidation (NIH funded). This
ongoing theme addresses the fundamental mechanisms of how vitamin
E protects cell membranes from oxidant stress. We have development
methods to monitor lipid radical formation in real time from intact,
functioning cells.
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Photodynamic Cancer Therapy (NIH funded). This research has been
an important theme in the lab for many years. We have demonstrated
mechanisms by which PDT produces free radicals in cells and tissues.
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Free radicals in myocardial ischemia reperfusion and defibrillation
injury (NIH funded). This project is being done with Dr. Richard Kerber's
research group. Dr. Mukesh Sharma won the American College of Cardiology
1992 Young Investigator Award with the results of this project.
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The role of metals in free radical oxidations. This has been a long
time area of research. We were the first to demonstrate that adventitious
iron can change the results observed in free radical experiments and
that chelating agents could be used to modulate the catalytic activity
of metals. This work has been honored as a Citation Classic. [Citation
Commentary] [Paper 1] [Paper 2]
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The chemistry of EPR spin trapping. This represents the ongoing developmental
aspects of the ESR Facility.
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UV light and free radical production in skin. Thus far, the results
of this project are the first to demonstrate directly UV light production
of radicals in skin and that antioxidants blunt this radical formation
with a concomitant reduction of skin tumor formation.
Example Publications
- Schafer FQ, Buettner GR. (2001) Redox state of the cell as viewed though the glutathione disulfide/glutathione couple. Free Radic Biol Med. 30:1191-1212. [Abstract][Article PDF][DOI Article]
- Wang M, Kirk JS,. Domann FE, Zhang HJ,. Schafer FQ, Weydert CJ, Spitz DR, Buettner GR, Oberley, LW. (2005) Manganese superoxide dismutase suppresses hypoxic induction of hypoxia inducible factor-1a and vascular endothelial growth factor. Oncogene, 24: 8154-8166. [Article PDF] [DOI Article]
- Buettner GR, Ng CF, Wang W, Rodgers VGJ, Schafer FQ. (2006) A new paradigm: Manganese superoxide dismutase influences the production of H2O2 in cells and thereby their biological state. Free Radic Biol Med. 41: 1338-1350. [Article PDF] [DOI Article]
- Vislisel JM, Schafer FQ, Buettner GR. (2007) A simple and sensitive assay for ascorbate using a plate reader . Analytical Biochemistry. 365: 31-39.
- Chen Q, Espey MG, Sun AY, Lee JH, Krishna MC, Shacter E, Choyke PL, Pooput C, Kirk KL, Buettner GR, Levin M. (2007) Ascorbic acid in pharmacologic concentrations: a pro-drug for selective delivery of ascorbate radical and hydrogen peroxide to extracellular fluid in vivo. Proc Natl Acad Sci USA. 104: 8749-8754.
Honors, Awards and Organizations
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President, 2004-2006, Society for Free Radical Biology and Medicine, the largest professional society devoted to the study of free radical and redox biology.
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Distinguished Service Award, 2006, Society for Free Radical Biology and Medicine
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Staples Distinguished Lecturer, The
University of Maine, 2000
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Master Teacher Award, 1999; given by the Oxygen
Society/Free Radical Research Society.
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Citation Classic selection by the Institute for Scientific Information, the publishers of Current Contents, for: Oberley LW and Buettner GR (1979) The role of superoxide dismutase in cancer: A review. Cancer Research 39: 1141-1149. [Citation Commentary] [Abstract] [PDF]
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Citation Classic selection by the Institute for Scientific Information, the publishers of Current Contents, for: Buettner GR, Oberley LW, and Leuthauser SWHC. (1978) The effect of iron on the distribution of superoxide and hydroxyl radicals as seen by spin trapping and on the superoxide dismutase assay. Photochem Photobiol 28: 693-695. [Citation Commentary] [PDF]
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Citation Classic selection by the Institute for Scientific Information, the publishers of Current Contents, for: Buettner GR and Oberley LW. (1978) Considerations in the spin trapping of superoxide and hydroxyl radicals in aqueous systems using 5,5-dimethyl-1-pyrroline-1-oxide. Biochem Biophys Res Commun 83: 64-74 [Citation Commentary] [PDF]
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1995-Present, Editorial Board Archives
Biochemistry and Biophysics
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1995-Present, Editorial Board Free
Radicals in Biology and Medicine
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1999-2004, Editorial Board Free Radical Research
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1985-1987, Fulbright Scholar GSF Research
Institute, Munich, Germany
Education
Post-Graduate Education
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Fulbright Scholar GSF Research Institute,
Munich, Germany, 1985-1987.
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NRSA Senior Fellow NIEHS, North Carolina, 1984-1985.
- NRSA Post Doctoral Fellow Radiation Research Lab, The University
of Iowa, 1976-1978
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